To avoid the loss of stationary phase, which shortens the column’s life time, it can be bound covalently towards the silica particles. Bonded stationary phases
각각 다른 산업 분야에 대한 자세한 정보 및 다양한 카테고리는 다음 써모 피셔 사이언티픽 학습 센터에서 산업 및 응용 과학 페이지를 확인하세요.
예를 들어 설탕과 같이 물에 녹기 쉬운 물질을 첨가했을 때 설탕은 기름층에 거의 녹지 않으므로 물층에 많이 존재하게 됩니다. 반대로 식용유와 같이 헥산에 녹기 쉬운 용질을 첨가했을 때는 물층보다 기름층에 많이 존재합니다. 이와같이, 설탕과 식용유는 물과 헥산의 두 상 사이의 존재의 비율(=분배 비율)이 크게 다르기 때문에, 만약 당신과 이 분액깔대기에서 설탕만을 분리하고 싶다면, 분액깔대기에서 물층만을 꺼내 물을 증류시키면 설탕만을 얻을 수 있습니다.
By subsequent these tips and systematically addressing opportunity will cause, it is possible to efficiently troubleshoot frequent HPLC complications and be certain your analyses are correct and reputable.
The data acquisition system documents and analyses the detector signals, enabling chemical substances being quantified dependent on their own peak locations within the chromatogram.
The figure down below reveals the calibration curve and calibration equation to the list of external criteria. Substituting the sample’s peak region into your calibration equation gives the concentration of caffeine inside the sample as 94.4 mg/L.
Given that the mobile period flows through the column, the compounds while in the sample connect with the stationary phase. This interaction brings about the compounds to different based on their certain Houses, which include polarity, dimension, charge, or affinity.
By diligently considering Each individual phase on the HPLC Evaluation approach, from sample preparation to information interpretation, laboratory staff can be certain precise, trustworthy, and efficient separation of factors in advanced mixtures.
-hydroxybenzoic acid—with get more info a nonpolar C18 column employing an aqueous buffer of acetic acid and sodium acetate since the cellular section. The retention moments for these weak acids are shorter when utilizing a much less acidic mobile period since Each and every solute is current within an anionic, weak foundation kind that's a lot less soluble in the nonpolar stationary stage.
To outcome a far better separation in between two solutes we have to Increase the selectivity aspect, (alpha). There are two frequent strategies for escalating (alpha): including a reagent to the mobile section that reacts While using the solutes in the secondary equilibrium response or switching to a distinct cell section.
The cell section’s move amount is set through the blended speeds of the two pumps. By changing the relative speeds of The 2 pumps, unique binary cellular phases may be prepared.
Just after placing the sample from the sample reservoir the injection process is entirely automatic. The injector injects click here the sample into the continually flowing cellular period stream that carries the sample to the HPLC column.
ノブをインジェクト側に切り替え、サンプルを流路に注入する。マニュアルインジェクターに電気信号を出力する機能が付いていれば、この時にインジェクション信号を検出器またはインテグレーターに送ることが出来る。
The choice to begin with acetonitrile is arbitrary—we could equally as effortlessly decide on to start with methanol or with tetrahydrofuran.